Excretion in rats

Sixteen rats with eight males and eight females were put into metabolic cages individually and then fasted overnight till 2 h after oral administration of harmaline and harmine at a dose of 40.0 mg/kg. Access to water was maintained all the time. Samples including blank urine and feces before dosing, urine and feces from 0 to 24 h, 24 to 48 h, and 48 to 72 h after dosing were collected and stored at −80°C until analysis.

Urine samples were thawed and an aliquot of 100 μL was processed for detection as described in Section Drug Analysis. Feces were homogenized and then weighed, added 6-folds of acetonitrile, extracted by ultrasonic wave for 1 h and then centrifuged. An aliquot of 100 μL was also processed for detection as described in Section Drug Analysis.