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Microbiota analysis

QH Quirine LM Habes
PK Prokopis Konstanti
HK Harmke D Kiers
RK Rebecca M Koch
RS Roeland F Stolk
CB Clara Belzer
MK Matthijs Kox
PP Peter Pickkers
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Subjects collected faecal samples at home 1 day before and 1 day and 7 days after the endotoxaemia experiment. These samples were stored at −20°C and transported to the study centre for storage at −80°C. Bacterial DNA was extracted using a bead‐beating protocol, and the extracted DNA was used for 16s rRNA gene sequencing (Illumina MiSeq sequencing) as described previously. 29 Data were processed using the NG‐Tax software package. 30

Copyright and License information:  ©2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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