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ELISA

JD Judit Danis
AG Anikó Göblös
ZB Zsuzsanna Bata-Csörgő
LK Lajos Kemény
MS Márta Széll
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Cell supernatants were centrifuged (5,000 rpm, 4 min, 4°C) to pellet cell debris, and the amount of cytokines IL-1α, IL-1β, IL-6, IL-8, CCL-5, and TNF-α was determined by ELISA (IL-1α Duo Set, IL-1β ELISA Duo Set, and TNF-α Duo Set, R&D Systems, Minneapolis, MN, USA; Human IL-6 Mini TMB ELISA Development Kit, Human IL-8 TMB ELISA Development Kit, Human RANTES ATBS ELISA Development Kit, PeproTech, Rocky Hill, NJ, USA), according to the manufacturer’s instructions.

Copyright and License information:  ©2017 Danis, Göblös, Bata-Csörgő, Kemény and Széll.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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