2.5. Transfection Using TurboFect or Lipofectamine Commercial Reagents

Lipofectamine 2000 (cationic lipid, Invitrogen) and TurboFect (cationic polymer, Fermentas) transfection reagents were employed to deliver pEGFP-Nef into DCs (1 × 10⁵ cell/well) and MSCs (2 × 10⁵ cell/well) in a 24-well plate. A FACSCalibur flow cytometer (Partec) was used to quantify the transfection efficiency at 72 hours posttransfection. The negative and positive controls were the untransfected cells and the transfected cells with pEGFP-N1 (Clontech, USA), respectively, [22].