Circular dichroism spectra (CD) analysis

CD was performed to analyze the changes that occurred in the secondary structure of HSA in different formulations. CD analysis was carried out on a Chirascan-Plus CD Spectrometer with a 0.1-cm quartz cell. The concentrations of HSA in free HSA solution, Abraxane suspension, and self-made nanoparticles suspension were all 0.8mg/mL in the present study. The measurements were taken in the UV region with a wavelength range of 180-260nm and a band width of 5nm, and each reported spectrum was the average of three scans [31, 32]. Secondary-structure calculations were performed by Jasco software [33].