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Chronic constriction injury (CCI) of the sciatic nerve
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The model was established according to the experimental method of Bennett and Xie22. The rats were abdominally injected with 50 mg/kg body weight sodium pentobarbital. Under anesthesia, the left sciatic nerve of each rat was exposed and separated at the mid-thigh level. The sciatic nerve was tied with four ligatures (4–0 chromic gut sutures) spaced 1 mm apart. 4–0 chromic gut sutures were used to suture the muscle, and wound clips were used to close the incision. Penicillin (4000 units) was intramuscularly injected into each rat.
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