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Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5], 20 mM KCl, 10 mM MgCl2, 10 mM β-mercaptoethanol, and 1.25 M sucrose). The reaction products were separated by SDS-PAGE and analyzed with a phosphorimager (Bio-Rad, Irvine, CA, USA).
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