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Myotube length and diameter measurement

TH Takaaki Higashihara
HN Hiroshi Nishi
KT Koji Takemura
HW Hiroshi Watanabe
TM Toru Maruyama
RI Reiko Inagi
TT Tetsuhiro Tanaka
MN Masaomi Nangaku
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C2C12 myoblast cells were seeded in a 6-well or 12-well plate and cultured in a differentiation medium as described above. When cells reached full differentiation after 5 days, the cultured medium was replaced with DMEM containing 4% bovine serum albumin (either with or without IS, clenbuterol (1 μM), L-ascorbic acid (100 μM) and N-Acetyl-L-cysteine (1 mM)) and cultured for 1 or 2 more days. The medium with IS and reagents were changed every day. Cell morphology was observed with microscopy (Olympus, Inc.). Myotube length and diameters were measured in at least 100 to 200 myotubes using ImageJ software (National Institute of Health (NIH), Bethesda, MD, USA).

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