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A GreenNuc™ Caspase-3 assay kit for Live Cells (cat. no., C1168S; Beyotime Institute of Biotechnology) was used to measure caspase-3 activity, according to kit instructions. After treated with NE for 24 h, single-cell suspensions were collected and added in 1 μl GreenNuc™ Caspase-3 substrate, mixed thoroughly and incubated for 20 min at 37°C in the dark. The levels of caspase-3 activity were determined by flow cytometry (Crowley and Waterhouse, 2016).
Copyright and License information: ©2021 Wang, Jing, Bi, Li, Ma, Yang and Zhang.
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