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Alkaline phosphatase (ALP) activity was used as a biomarker of osteogenic differentiation in VSMCs. ALP activity was assessed colorimetrically using an ALP Activity Assay kit (Nanjing Jiancheng Chemical Industrial Co., Nanjing, China), and normalized to total protein using the Bradford method [25]. Lysates were prepared according to the manufacturer’s instructions.
Copyright and License information: ©2016 Liu et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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