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BioID-WB

MB Melusine Bleu
FM Fanny Mermet-Meillon
VA Verena Apfel
LB Louise Barys
LH Laura Holzer
MS Marianne Bachmann Salvy
RL Rui Lopes
IB Inês Amorim Monteiro Barbosa
CD Cecile Delmas
AH Alexandra Hinniger
SC Suzanne Chau
MK Markus Kaufmann
SH Simon Haenni
KB Karolin Berneiser
MW Maria Wahle
IM Ivana Moravec
AV Alexandra Vissières
TP Tania Poetsch
EA Erik Ahrné
NC Nathalie Carte
JV Johannes Voshol
EB Elisabeth Bechter
JH Jacques Hamon
MM Marco Meyerhofer
DE Dirk Erdmann
MF Matteo Fischer
TS Therese Stachyra
FF Felix Freuler
SG Sascha Gutmann
CF César Fernández
TS Tobias Schmelzle
UN Ulrike Naumann
GR Guglielmo Roma
KL Kate Lawrenson
CN Cristina Nieto-Oberhuber
AC Amanda Cobos-Correa
SF Stephane Ferretti
DS Dirk Schübeler
GG Giorgio Giacomo Galli
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IGROV-1-Cas9-PAX8-BioID-T2A-mCherry Clone B3 and C5 cells were seeded at 4 × 105 cells/well in a 6-well plate. The following day, cells were treated with 50 μM biotin (Fluka) or dimethyl sulfoxide (Sigma) for 24 h. Cell pellets were harvested and lysed in RIPA buffer supplemented with protease inhibitor cocktail (Roche). Lysates (80 μg) were then incubated with streptavidin beads (Cell Signaling) overnight under rotation at 4 °C. Immunoprecipitates were washed five times with RIPA buffer containing protease inhibitors, eluted with NuPAGE Blue Sample buffer (Life Technologies) by incubation at 95 °C for 10 min, and resolved by standard SDS‐PAGE gel electrophoresis and Western blotting as described above.

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