Phytochemical Screening

In order to identify the phytochemicals in the extract (flavonoids, carbohydrates, saponins, glycosides, tannins, alkaloids, and proteins), a phytochemical analysis was performed. Briefly, Dragondroff’s reagent, Mayer’s reagent, Hager’s reagent, and Wagner’s reagent were used to detect the alkaloids. Lead acetate test, Ferric chloride test, Froth test, potassium hydroxide test, Ninhydrin test, Biuret test, Anthraquinone glycoside test, Molisch’s and Benedict’s test, Libermann-Burchard reaction, Phenol test, Salkowski’s and LibermannBurchard’s test were used to detect the flavonoids, tannins, saponins, fixed oil and fats, proteins and amino acid, glycosides, reducing sugars, steroids, phenols, diterpenes, and photosterols, respectively (Rahman, 2013; Tiwari et al., 2011).