Cells were seeded in FGM at 10,000 cells/well in 24 well plates, cultured overnight and then serum-deprived for 72 h prior to re-addition of FGM plus inhibitors of ATM (KU55933, 10 μM), ATR (AZ20, 10 μM), DNA-PK (NU7026, 3 μM) (all Generon) or DMSO vehicle.
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