2.5. Conditioned taste aversion experiments

All behavioral testing was conducted during the dark phase. Mice were separated and housed one and one a minimum of 5 d prior to experimental onset. Days 1–7, mice were habituated to water deprivation for 4 h a day. Day 8 (conditioning day), mice were water deprived for 4 h, then given access to a saccharin solution (0.15%) for 1 h, and immediately thereafter given an injection of either LPS or saline. Days 9–10 mice were only exposed to water deprivation. At the test day (Day 11) mice were water deprived for 4 h and then given saccharin solution for 1 h after which saccharin intake was measured. Pretreatment with SC-560 and parecoxib was given 1 h before LPS injection on the conditioning day (Day 8), i.e. at the same time mice got access to saccharin. The timing for administration of these inhibitors is in concordance with the timing of administration in the food intake studies.