Oil red O for Lipid Staining

The adipocytes were fixed with 4% paraformaldehyde for 30 min and washed three times with phosphate buffered saline (PBS). Next, the adipocytes were stained with 0.5% Oil Red O for 30 min, followed by washing with PBS. Images were captured at 100 or 200 × magnification under a light microscope. For quantitative analysis, isopropyl alcohol was added to dissolve the Oil Red O, and the optical density value was measured by spectrophotometer at 520 nm. The experiment was repeated for 6 times.