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Western-Blot Analysis of Histone Modifications

JF Jing Feng
DC Donghong Chen
AB Alexandre Berr
WS Wen-Hui Shen
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Histone-enriched nuclear protein extracts were prepared from 2-week-old plants of the wild type and the atbmi1a atbmi1b and atzrf1a-1 atzrf1b-1 mutants according to a previously described protocol (Yu et al., 2004). Proteins were analyzed on SDS-PAGE gels and detected using different antibodies specifically recognizing histone H3 (catalog no. 05-499; Millipore), H3K4me3 (catalog no. 07-473; Millipore), H3K27me3 (catalog no. 07-449; Millipore), H3K36me3 (catalog no. Ab9050; Abcam), H2Aub1 (catalog no. 8240; Cell Signaling), or ubiquitin (catalog no. FK-2; Upstate Millipore).

Copyright and License information:  ©2016 American Society of Plant Biologists. All Rights Reserved.
http://www.aspb.org/publications/aspb-journals/open-articles.

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