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Binding Nuclei to Concanavalin A-Coated Magnetic Beads (Con-A Beads)

WO Weizhi Ouyang
XZ Xiwen Zhang
YP Yong Peng
QZ Qing Zhang
ZC Zhilin Cao
GL Guoliang Li
XL Xingwang Li
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Wash the nuclei pellet twice with 500 μl ice-cold Wash Buffer. Centrifuge in a swinging bucket rotor at 600 g for 3 min at 4°C; discard Wash Buffer.

Wash 20 μl Con-A beads with 500 μl Binding Buffer twice to activate Con-A beads. Place the tube on a magnet stand and remove the liquid.

Resuspend Con-A beads with 100 μl Binding Buffer. Add the activated beads to isolated nuclei and incubate the mixture at 4°C for 15 min.

Copyright and License information:  ©2021 Ouyang, Zhang, Peng, Zhang, Cao, Li and Li.
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