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Quantification of Serum IL-6 and TNF-α by ELISA Assays
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The levels of serum interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) were quantified using the mouse IL-6 (E03I0006) and TNF-α (E03T0008) ELISA kits according to the manufacturer’s instructions (BlueGene, Shanghai, China). The sample absorbance was measured in a microplate reader (Multiskan MK3; Thermo Fisher Scientific, Waltham, MA, United States).
Copyright and License information: ©2021 Xu, Huang, Xu, He, Zhao, Peng, Zhao, Huang, Cao, Zhou, Zhou and Nie.
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