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2.6. Cell Count and Vitality: Acridine Orange/DAPI Double Staining

MV Milena Villarini
MA Mattia Acito
RV Raffaella di Vito
SV Samuele Vannini
LD Luca Dominici
CF Cristina Fatigoni
RP Rita Pagiotti
MM Massimo Moretti
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The number of total and viable cells was estimated by staining cell populations with acridine orange (AO) and DAPI fluorophores. After cells treatment, aliquots of cell suspensions were loaded into Via1-Cassette. The inside of the Via1-Cassette is coated with AO (staining the entire population of cells) and DAPI (staining the non-viable cells). The Via1-Cassettes were then placed in a NucleoCounter® NC-3000™ (Chemometec, Allerød, Denmark) cytometer where cell concentration and viability were determined. Total cell concentration in Via1-Cassette was displayed on a personal computer using the NucleoView software. Viability of treated cells was calculated as a percent of control.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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