Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
4.6. Isolation and Culture of Fresh Mouse Proximal Tubular Epithelial Renal Cells
Request a Protocol
Ask a
question
Favorite
Primary cells were isolated from the kidneys of wild-type (WT) mice and cultured as previously described [33] with minor modifications. The cells were incubated at 37 °C in humidified 5% CO2 in air, and the medium was changed every 6 days until the cells reached 60% confluence.
Copyright and License information: ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question0 Q&A
