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Analysis of total anthocyanin
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Fresh leaf tissue (0.2 g) was collected for the measurement of anthocyanin content. In brief, anthocyanins were extracted in 10 ml acidified methanol (99 CH3OH:1HCl, v/v) at 4 °C for 24 h in the dark, clarified by centrifugation at 12,000 × g for 2 min, and the absorbance of supernatants was determined using a UV-visible spectrophotometer (UV-2550, Shimadzu, Japan) from optical density (OD) at 530 and 600 nm. Anthocyanin concentrations were expressed as U∙g-1, where U was calculated as (OD530–OD600)/0.1 [18].
Copyright and License information: The Author(s). ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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