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4.6. Cell Cultures

BD Barbara Dyba
ER Elżbieta Rudolphi-Szydło
AB Anna Barbasz
AC Agnieszka Czyżowska
KH Konrad Kamil Hus
VP Vladimír Petrilla
MP Monika Petrillová
JL Jaroslav Legáth
AB Aleksandra Bocian
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U-937 (Human histiocytic lymphoma cell line) and HL-60 (human promyelocytic cells line) (both purchased from ATCC) were cultured in suspension in RPMI 1640 (Cyto Gen GmbH); U-937 contained5% bovine serum (FBS), while HL-60 contained 10% FBS. A 0.01% penicillin-streptomycin mixture was applied to both cultures. After incubation at 37 °C/24 h in a humidified atmosphere, cells were used for the experiments.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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