2.1. Materials

Penicillin-Streptomycin (10,000 U/mL) antibiotics (Gibco, 15140122), Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, 23227), Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, 11965092), Fetal Bovine Serum (FBS) (Gibco, 10270106), and Opti-MEM^TM Reduced Serum Medium (Gibco, 31985062) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Hexadimethrine bromide (polybrene, 107689), puromycin dihydrochloride (>98%, P9620), 4′,6′-diamidino-2-phenylindole (DAPI, 10236276001), and doxorubicin were purchased from Sigma-Aldrich (St. Louis, MI, USA). Nucleic Acid Gel Stain (YeaRed, 10202ES76), Agarose (10208ES76), Cell Counting (CCK-8) Kit (40203ES76), total RNA extraction reagent (Trizol, 10606ES60), first Strand cDNA Synthesis Kit (11121ES60), and Hieff™ qPCR SYBR^® Green Master Mix (11203ES08) were purchased from Yeasen Biotech (Shanghai, China). Tris base-acetic acid- EDTA (TAE) buffer (ST716), DNA loading (D0071), and Relilla luciferase reporter kit (RG017) were acquired from the Beyotime company (Shanghai, China).