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MDSC culture and maintenance

DC David Kung-Chun Chiu
AT Aki Pui-Wah Tse
IX Iris Ming-Jing Xu
JC Jane Di Cui
RL Robin Kit-Ho Lai
LL Lynna Lan Li
HK Hui-Yu Koh
FT Felice Ho-Ching Tsang
LW Larry Lai Wei
CW Chun-Ming Wong
IN Irene Oi-Lin Ng
CW Carmen Chak-Lui Wong
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Freshly isolated splenic MDSCs or bone marrow progenitors were cultured in RPMI-1640 medium supplemented with 10% FBS, 10 ng ml−1 granulocyte–macrophage colony-stimulating factor (R&D Systems), 10 ng ml−1 interleukin-4 (R&D Systems), and 50 µM 2-mercaptoethanol (Sigma-Aldrich), alone or in the presence of 100 µM 5′-AMP (Sigma-Aldrich), 100 µM APCP (Tocris Bioscience), 100 µM adenosine (Sigma-Aldrich), 100 µM NECA (Tocris Bioscience), or HCC cell CM.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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