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2.5.2. Crystal Violet Assay for Cell Number
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Cells were fixed in 10% formalin (30 min), washed and stained with crystal violet (0.5% w/v) for 30 min, and washed again. Cells were visualised, or the crystal violet was then dissolved in 10% acetic acid and absorbance (600 nm) using a GloMax plate reader (Promega, Madison, WI, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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