Data are presented as mean ± SEM of at least three independent replicates. Statistical analysis was performed with GraphPad Prism 6.07 (GraphPad Software Inc., San Diego, CA, USA). The D’Agostino-Pearson normality test was used to test the data sets for normal distribution. Statistical significance was tested by using an unpaired t-test or ordinary one-way ANOVA test with Dunnett’s multiple comparisons test. p-values < 0.05 were considered statistically significant, with * = p < 0.05, * = p < 0.01, and *** = p < 0.001.
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