5.3.2. Biodegradation Rate of MC-LR in Complex Mixtures

To compare MC-LR biodegradation rate by P. toxinivorans in a more complex chemical mixture, 10 μg/mL of this variant (in 5 µL) was added to OD-corrected bacteria (5 µL) in a mixture of cyanopeptides and in a M. aeruginosa crude extract diluted in R2A media (140 µL). The cyanopeptide mix consisted of all cyanopeptides previously tested (DM-LR, MC-RR, MC-LF, MC-YR, ANBP-A, ANBP-B, AC-A, and AC-D), which was prepared in R2A media (140 µL) to achieve a final concentration of 1 µg/mL. M. aeruginosa (PCC 7806) crude extract was obtained from a supernatant produced by freezing–thawing (−20 °C) and centrifuged over 15 min at 13,000 × g. The amount of MC-LR present in the resulting supernatant of the crude extract was quantified through UPLC–PDA–MS at 2.45 µg/mL. Then, it was diluted 1:10 in R2A media and 140 µL were added within each well with bacteria (5 µL) and 10 µg/mL MC-LR (5 µL). All conditions were as before (Section 5.3) with samples taken at 0, 3, and 7 days of incubation.