3.5.3. Quantification of Cell Number and Viability

For the assessment of total cell numbers and viability within individual experimental groups, floating and adherent cells were harvested after treatment with the studied derivatives and evaluated using a Bürker chamber with eosin staining. A549 cells were plated at a density of 135 × 10³ cell/well into a 6-well plate and treated with different concentrations of derivatives (35 and 65 µM) for 24 hrs. The total cell number was expressed as a percentage of the total cell number of the untreated control. Viability was expressed as a percentage of viable, eosin negative cells. The results are presented as the average percentage of cells from three independent experiments.