2.2. Physicochemical Parameters Prediction of Foreign Peptides

The physical and chemical properties of the peptides were calculated using the ProtParam program on the ExPASy server (http://web.expasy.org/protparam/; accessed on 8 January 2012). These computed parameters include the theoretical pI, number of charged residues and grand average of hydropathicity (GRAVY; positive GRAVY index, hydrophobic; negative GRAVY index, hydrophilic). The computational predictions of disulfide bond connectivity were estimated using the DiANNA 1.1 web server [25] to predict hypothetical chimeric CP disulfide bonds. Protein secondary structures were simulated using the PSIPRED protein structure prediction server [26].