Double beam Analykjena UV-visible spectrophotometer (Model 205, Germany) was used to measure the absorbance of drug solution. pHmeter (Serial no.- 453088, Hanna, Portugal), electronic balance (Serial no.- 1508, OHAUS, Germany), metabolic shaking incubator (Serial no.- 490639525, New Brunswick Scientific, USA), micro syringe (Serial no.- HN42250, Jencons, UK), hot plate (Serial no.- SWT.550010W, Gallenkamp, England) were used in this study.
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