2.6. Intracerebroventricular (ICV) cannulation

Mice were anesthetized with isoflurane (5% for induction; 1%–2% to sustain). Once anesthetized, each mouse was placed in a stereotaxic device and implanted with a stainless-steel cannula (25G, 9 mm length) into the lateral brain ventricle (0.3 mm posterior and 1.0 mm lateral to bregma and 3.0 mm below the surface of the skull). Mice were allowed to recover for 7–10 days.