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Bafilomycin A1 (Baf-A1)-treatment and autophagic flux analyses

CD Cajsa Davegårdh
JS Johanna Säll
AB Anna Benrick
CB Christa Broholm
PV Petr Volkov
AP Alexander Perfilyev
TH Tora Ida Henriksen
YW Yanling Wu
LH Line Hjort
CB Charlotte Brøns
OH Ola Hansson
MP Maria Pedersen
JW Jens U. Würthner
KP Klaus Pfeffer
EN Emma Nilsson
AV Allan Vaag
ES Elisabet Stener-Victorin
KP Karolina Pircs
CS Camilla Scheele
CL Charlotte Ling
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Primary myoblasts were treated with 100 nM Baf-A1 for 3 h per day during 4 consecutive days, starting when the culture medium was switched to differentiation medium 1. For autophagic flux analyses, primary myoblasts (day 3 of differentiation) were treated with 100 nM Baf-A1 for 3 h under both basal condition (standard culture media, differentiation medium 2), and serum- and amino acid-free starvation condition (HBSS). Control cells were treated with vehicle only (1 μl/mL DMSO). The treatments did not affect cell viability, as determined by cell number (see Supplementary Fig. 2m–n).

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