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Dynamic light scattering (DLS)

MG Manuel Giménez-Andrés
TE Tadej Emeršič
SA Sandra Antoine-Bally
JD Juan Martin D'Ambrosio
BA Bruno Antonny
JD Jure Derganc
AČ Alenka Čopič
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Measurements of the mean hydrodynamic radius of the Plin4-oil droplets by dynamic light scattering were performed on a sample taken from the middle of the tube, avoiding any unreacted oil that remained at the top of the emulsion, at least 3 hr after vortexing to prevent the interference of gas bubbles with the measurement. Subsequent samples at later time points were removed in the same manner without any additional mixing. Emulsion samples were diluted 100-fold in freshly degassed HKM buffer with 1 mM DTT. Measurements were performed on a Zetasizer Nano ZS machine (Malvern) at 25°C, and data were processed using the CONTIN method.

Copyright and License information:  ©2021, Giménez-Andrés et al
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