NeuN-positive neuronal nuclei counting

YW Yingxi Wu
HW Haijian Wu
JZ Jianxiong Zeng
BP Brock Pluimer
SD Shirley Dong
XX Xiaochun Xie
XG Xinying Guo
TG Tenghuan Ge
XL Xinyan Liang
SF Sudi Feng
YY Youzhen Yan
JC Jian-Fu Chen
NM Naomi Sta Maria
QM Qingyi Ma
FG Fernando Gomez-Pinilla
ZZ Zhen Zhao
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For quantification, NeuN-positive neurons were quantified by using the Image J Cell Counter analysis tool. In each animal, 5 randomly selected fields from the cortex were analyzed in 4 nonadjacent sections (~ 100 µm apart). The number of NeuN-positive neurons colocalized with TUNEL was counted followed by the same procedure.

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