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Flow Cytometric Analysis of Cell Apoptosis
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A flow cytometric apoptosis kit was used to detect the apoptosis rate of chondrocytes across multiple intervention groups. The collected chondrocytes were resuspended in Annexin V-binding buffer and then stained with 5 µl phycoerythrin (PE)-labeled Annexin V and 5 µl 7-aminoactinomycin D (7-ADD) for 15 min at 37°C in the dark. The stained cells were assessed with a FACSAria™ II flow cytometer (BD Biosciences) within 1 h of staining.
Copyright and License information: ©2021 Zhou, Li, Wang, Jiang, Li, Wang, Wang, Ma and Cao.
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