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O. formigenes isolation and culturing
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O. formigenes strain PH1 was isolated from the stool sample of a primary hyperoxaluria patient using methods described in Allison et al., 1985. Both strains were cultured in defined oxalate broth as described (Allison et al., 1985), except that the concentration of oxalate is 50 mM. O. formigenes was cultured at 37°C in an anaerobic chamber before each mouse gavage.
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