2.7. ERK phosphorylation

Cells were starved for 2 hrs before treatment with vehicle or quinpirole (1 μM) for 5, 10 and 15 mins followed by solubilization in the buffer containing the Protease and Phosphatase Inhibitor Cocktail. Western blotting was performed to assess the level of phosphorylated ERK (pERK) by rabbit anti-pERK antibody (#9101, Cell Signaling) followed by secondary goat anti-rabbit IgG-HRP. Membranes were then stripped and total ERK (tERK) was probed with mouse anti-ERK (#4696, Cell Signaling) followed by secondary goat anti-mouse IgG-HRP. The level of pERK was normalized to total ERK and represented as relative to its own vehicle treatment.