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Analysis of hypothalamic–pituitary–gonadal hormones

JR J Rohayem
DH D Haffner
JC J F Cremers
SH S Huss
JW J Wistuba
DW D Weitzel
SK S Kliesch
KH K Hohenfellner
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Hormones of the hypothalamic–pituitary–gonadal (HPG) axis were analysed in serum from blood samples drawn between 8.00 and 12.00 a.m., including LH, FSH, testosterone and inhibin B. Serum LH and FSH levels were determined using highly specific time-resolved fluoro-immunoassays (Autodelfia, Freiburg, Germany). Serum testosterone was measured by fluorescence photometry (Architect ABBOTT GmbH, Wiesbaden, Germany) and inhibin B by a solid phase sandwich assay (Beckman-Coulter, Krefeld, Germany).

Copyright and License information:  ©2021-04-05 The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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