Correlation analysis was conducted for DEMs and genes using the COR program in R (Chong and Xia, 2018). The positively related genes with Pearson's correlation coefficients (PCC) >0.9 were selected and further filtered on the basis of gene length exceeding 750 bp.
The selected genes were then analyzed using the MapMan software package (http://mapman.gabipd.org/web/guest/home), which includes two freely available programs (Schwacke et al., 2019). First, each input gene was given a function annotation item “Bin” based on the reference database in MapMan. The pathway interpretation of these genes was then visualized in MapMan. After that, network visualization between the target metabolites and genes was completed using the Cytoscape software package (Shannon et al., 2003).
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