Synthesis of Sugar Laurate Esters

D-glucose and D-xylose laurate esters were synthesized using 1% lipase N435 from Novozymes in 2M2B in the presence of lauric acid or methyl laurate at 100 or 300 mM. After lyophilization, the wheat bran hydrolysate (see section “Wheat Bran Hydrolysis”) or the aqueous phase recovered from transglycosylation reaction (see section “Synthesis of Pentyl Xylosides”) was added in the acylation reaction mixture in order to obtain a total D-glucose and D-xylose concentration of 100 mM. Molecular sieves 3 Å or 5 Å were present at 10% (w/v), respectively, for reactions conducted with lauric acid or methyl laurate. Reactions (5 mL) were carried out in triplicates at 50°C under magnetic stirring (400 rpm) during 48 h.