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The different strains and clones of T. cruzi were cultured in LIT medium for 8 days at 28°C using an initial density of 1x106 parasites/mL. Growth was measured daily by counting the number of parasites using a Neubauer hemocytometer chamber. Counts were performed in triplicate.
Copyright and License information: ©2021 Ibarrola-Vannucci et al
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