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A549 and NCI-H1975 cell migration was determined in 6-well plates (105 cells/well) using a wound healing assay, as previously described (23). Briefly, the cells were used in the wound healing assay when the confluency of the cells was closed to 100%. After the cells were scratched using 10 μl pipette tips, the medium was replaced with FBS-free medium. The images were captured at 0, 24 and 48 h using an inverted fluorescence microscope (Olympus Corporation; magnification, ×100).
Copyright and License information: ©2021 Li et al.
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