Elevated zero maze

Anxiety-like behavior was assessed using an elevated zero maze [18] consisted of a ring-shaped platform, 60 cm in diameter, 4.3 cm wide, and elevated 52 cm above the floor. The maze was divided into four equal quadrants, two opposing arms were enclosed by 15.5 cm high gray walls whereas the other two were opened. Each mouse was placed in the center of an open arm and allowed to explore the platform for 5 min. Mouse behavior was recorded with an overhead video camera connected with the ANY-maze software for measuring the time spent and distance traveled in the open arm.