Whole-body CS exposure was performed as described [7]. Mice, starting at 6 weeks of age, were exposed to the smoke of 3R4F reference cigarettes (University of Kentucky, Lexington, KY) for 5 h per day. Mice receiving CS were gradually brought up to the target exposure over a period of 2 weeks, and treated 5 days/week for 6 weeks. Treatment was administered by placing mice in a Plexiglas smoking chamber (Teague Enterprises, Davis, CA). Smoke exposure was standardized to total suspended particles = 90 mg/m3, 11% mainstream and 89% sidestream smoke in the chamber of the machine. “Nonsmoking” (NS) treatment groups were conducted for the same periods of time, but mice were exposed to filtered room air.
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