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Stability of SARS-CoV-2 in various substrates

VB Velmurugan Balaraman
BD Barbara S. Drolet
DM Dana N. Mitzel
WW William C. Wilson
JO Jeana Owens
NG Natasha N. Gaudreault
DM David A. Meekins
DB Dashzeveg Bold
JT Jessie D. Trujillo
LN Leela E. Noronha
JR Juergen A. Richt
DN Dana Nayduch
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Ten individual cotton wicks (approx. 0.25 cm3 in size) were placed into separate wells of four different 12-well tissue culture plates (Corning). Two ml of three different substrates were added to six wicks and 2 ml of control substrate was added to four wicks. The final concentration of virus was 105 TCID50 per well. The plates were incubated in an environmental chamber at 22 °C and 60% relative humidity. The substrates were collected by adding 1 ml of DMEM growth medium onto the wicks and then, using a micropipettor, the medium was pipetted up and down 5 times before collection. The samples were collected at 0, 1, 4 and 24 h post-inoculation from the plates and stored at − 80 °C until analyzed. The virus titer of each sample was determined by performing the TCID50-cytopathic effect (CPE) assay (see below).

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