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HEK293T cells were transfected and anti-FLAG M2 magnetic beads (Sigma, M8823, United States; 50 μl/sample) were used for immunoprecipitation including rabbit anti-MDM2, rabbit anti-p53, rabbit anti-FXR, and rabbit anti-ubiquitin (Supplementary Table 7).
In another assay, the supernatants of Lenti-FXR CaSki cells were incubated with 2 μg IgG or mouse anti-SHP (Santa Cruz, CA, United States). The mixture was incubated with protein G-Agarose beads (Roche, United States) and the samples were assessed by western blot.
Details are shown in the Supplementary Materials.
Copyright and License information: ©2021 Huang, Wang, Chen, Zhong, Gao, Zhang, Niu, Li and Shi.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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