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BVL or BVL-7 was methylated by adding 1:1 hexane and 14% boron trifluoride/methanol and heated at 100°C for an hour. Fatty acids methyl esters were analyzed by a fully automated 6890N Network Gas Chromatography equipped with a flame-ionization detector (Agilent Technologies, Palo Alto, CA). Individual fatty acid was determined by retention time using a reference standard, GLC461 (Nu-Chek Prep, Elysian, MN).
Copyright and License information: ©2021 Chen, Su, Li, Liu, Pan, Cao and Kang.
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