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The 96-well plates were coated with cold Matrigel (Corning, Bedford, MA, USA). After incubation for 1 h at 37°C and 5% CO2, HUVEC (2 × 104 cells/well) were seeded in Matrigel-coated wells to produce the following groups: Control; 0.1% DMSO; 40 nM BRU; 10 μM tBHQ. Five fields of view were randomly selected at 100× magnification. The ImageJ software (National Institutes of Health, Bethesda, MD, USA) was used to determine the number of tubes formed in each group.
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