Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.10. Virus isolation and cell cultures

MB Michele Biagioli
SM Silvia Marchianò
RR Rosalinda Roselli
CG Cristina Di Giorgio
RB Rachele Bellini
MB Martina Bordoni
AG Anna Gidari
SS Samuele Sabbatini
DF Daniela Francisci
BF Bianca Fiorillo
BC Bruno Catalanotti
ED Eleonora Distrutti
AC Adriana Carino
AZ Angela Zampella
GC Gabriele Costantino
SF Stefano Fiorucci
request Request a Protocol
ask Ask a question
Favorite

The experiments were performed in Biological Safety Level 3 (BSL-3) virology laboratory of “Santa Maria della Misericordia” Hospital, Perugia, Italy. The SARS-CoV-2 strain was isolated as previously described [30]. For viral propagation, Vero E6 cell line was maintained in Eagle minimal essential medium (MEM) supplemented with 10% foetal bovine serum (FBS) and 100 U/mL penicillin–streptomycin solution. After isolation, SARS-CoV-2 titer was determined by Median Tissue Culture Infectious Dose (TCID50) endpoint dilution and stock aliquots were stored at −80 °C. The stock virus titer was 4.22 × 107 TCID50/mL.

Copyright and License information:  ©2021 Elsevier Inc. All rights reserved.
Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A