Mice

AV A. Vuorela
TF T. L. Freitag
KL K. Leskinen
HP H. Pessa
TH T. Härkönen
IS I. Stracenski
TK T. Kirjavainen
PO P. Olsen
OS O. Saarenpää-Heikkilä
JI J. Ilonen
MK M. Knip
AV A. Vaheri
MP M. Partinen
PS P. Saavalainen
SM S. Meri
OV O. Vaarala
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Homozygous HLA-DQA1*0102, -DQB1*0602, human CD4-transgenic Ab0 NOD mice (“HLA-DQ6.2 mice”; stock number 006023) were purchased from Jackson Laboratories, Bar Harbor, ME, USA, and bred at the University of Helsinki Laboratory Animal Centre17. All mice were housed under specific pathogen-free conditions, under a 14–10 h light–dark cycle, at 22 ± 2 °C and 50–60% humidity. Pandemrix vaccine was injected under isoflurane anesthesia into the right thigh muscle (50 μl emulsion volume; male or female mice; 3 months of age), followed by one booster injection after 14 days. After 6–9 weeks from the first injection, mice were exsanguinated by retro-orbital bleeding under ketamine/xylazine anesthesia (Intervet International, Boxmeer, The Netherlands; Bayer HealthCare, Leverkusen, Germany), and spleens were harvested for collection of cells.

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